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10.1074/jbc.M116.729731 http://scihub22266oqcxt.onion/10.1074/jbc.M116.729731 C5034030!5034030 !27528603     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27528603 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2016 ; 291 (39 ): 20295-20302 Nephropedia Template TP {{tp|p=27528603 |t=2016. Activated G Protein G?s Samples Multiple Endomembrane Compartments |pdf=|usr=}}{{27528603 }} gab.com Text Activated G Protein G s Samples Multiple Endomembrane Compartments JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27528603 #FOAvip Heterotrimeric G proteins are localized to the plasma membrane where they transduce extracellular signals to intracellular effectors. G proteins also act at intracellular locations, and can translocate between cellular compartments. For example, G?s can leave the plasma membrane and move to the cell interior after activation. However, the mechanism of G?s translocation and its intracellular destination are not known. Here we use bioluminescence resonance energy transfer (BRET) to show that after activation, G?s rapidly associates with the endoplasmic reticulum, mitochondria, and endosomes, consistent with indiscriminate sampling of intracellular membranes from the cytosol rather than transport via a specific vesicular pathway. The primary source of G?s for endosomal compartments is constitutive endocytosis rather than activity-dependent internalization. Recycling of G?s to the plasma membrane is complete 25 min after stimulation is discontinued. We also show that an acylation-deacylation cycle is important for the steady-state localization of G?s at the plasma membrane, but our results do not support a role for deacylation in activity-dependent G?s internalization. Twit Text FOAVip Activated G Protein G s Samples Multiple Endomembrane Compartments ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27528603 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27528603 #FOAvip English Wikipedia <ref>{{Cite pmid|27528603 }}</ref> Activated G Protein G?s Samples Multiple Endomembrane Compartments #MMPMID27528603 Martin BR ; Lambert NA J Biol Chem 2016[Sep]; 291 (39 ): 20295-20302 PMID27528603 show ga Heterotrimeric G proteins are localized to the plasma membrane where they transduce extracellular signals to intracellular effectors. G proteins also act at intracellular locations, and can translocate between cellular compartments. For example, G?s can leave the plasma membrane and move to the cell interior after activation. However, the mechanism of G?s translocation and its intracellular destination are not known. Here we use bioluminescence resonance energy transfer (BRET) to show that after activation, G?s rapidly associates with the endoplasmic reticulum, mitochondria, and endosomes, consistent with indiscriminate sampling of intracellular membranes from the cytosol rather than transport via a specific vesicular pathway. The primary source of G?s for endosomal compartments is constitutive endocytosis rather than activity-dependent internalization. Recycling of G?s to the plasma membrane is complete 25 min after stimulation is discontinued. We also show that an acylation-deacylation cycle is important for the steady-state localization of G?s at the plasma membrane, but our results do not support a role for deacylation in activity-dependent G?s internalization. |Acylation [MESH] |Bioluminescence Resonance Energy Transfer Techniques/methods [MESH] |Chromogranins/genetics/*metabolism [MESH] |Endocytosis/*physiology [MESH] |Endoplasmic Reticulum/*enzymology/genetics [MESH] |Enzyme Activation/physiology [MESH] |GTP-Binding Protein alpha Subunits, Gs/genetics/*metabolism [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Intracellular Membranes/*enzymology [MESH]Activated G Protein G?s Samples Multiple Endomembrane Compartments (epmc).pdf DeepDyve Pubget Overpricing