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2017 ; 12
(2
): ä Nephropedia Template TP
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A versatile modular bioreactor platform for Tissue Engineering
#MMPMID27492568
Schuerlein S
; Schwarz T
; Krziminski S
; Gätzner S
; Hoppensack A
; Schwedhelm I
; Schweinlin M
; Walles H
; Hansmann J
Biotechnol J
2017[Feb]; 12
(2
): ä PMID27492568
show ga
Tissue Engineering (TE) bears potential to overcome the persistent shortage of
donor organs in transplantation medicine. Additionally, TE products are applied
as human test systems in pharmaceutical research to close the gap between animal
testing and the administration of drugs to human subjects in clinical trials.
However, generating a tissue requires complex culture conditions provided by
bioreactors. Currently, the translation of TE technologies into clinical and
industrial applications is limited due to a wide range of different
tissue-specific, non-disposable bioreactor systems. To ensure a high level of
standardization, a suitable cost-effectiveness, and a safe graft production, a
generic modular bioreactor platform was developed. Functional modules provide
robust control of culture processes, e.g. medium transport, gas exchange,
heating, or trapping of floating air bubbles. Characterization revealed improved
performance of the modules in comparison to traditional cell culture equipment
such as incubators, or peristaltic pumps. By combining the modules, a broad range
of culture conditions can be achieved. The novel bioreactor platform allows using
disposable components and facilitates tissue culture in closed fluidic systems.
By sustaining native carotid arteries, engineering a blood vessel, and generating
intestinal tissue models according to a previously published protocol the
feasibility and performance of the bioreactor platform was demonstrated.