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Virol+J
2015 ; 12
(?): 73
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A transgenic ginseng vaccine for bovine viral diarrhea
#MMPMID25948546
Gao Y
; Zhao X
; Sun C
; Zang P
; Yang H
; Li R
; Zhang L
Virol J
2015[May]; 12
(?): 73
PMID25948546
show ga
BACKGROUND: Bovine viral diarrhea virus (BVDV) infections are endemic in cattle
populations worldwide and cause major economic losses. Thus, an effective vaccine
is needed against the transmission of BVDV. The glycoprotein E(rns) is one of the
envelope proteins of this virus and shows BVDV-related immunogenicity. Here, we
report the use of Panax ginseng as an alternative production platform for the
expression of glycoprotein E(rns) via Agrobacterium-mediated transformation.
RESULT: Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR
analyses showed that pBI121-E(rns) was stably integrated into the chromosome of
transformants. ELISA assay and Western blot analysis confirmed the antigenicity
of plant-derived E(rns) glycoprotein. Immunogenicity was evaluated subcutaneously
in deer using a soluble protein extract of dried transgenic ginseng hairy roots.
Specific humoral and cell-mediated immune responses against BVDV were detected
following immunization. CONCLUSION: These results demonstrated that the E(rns)
glycoprotein could be expressed in ginseng hairy roots and that plant-derived
glycoprotein E(rns) retained its antigenicity and immunogenicity.
|Animals
[MESH]
|Antibodies, Viral/blood
[MESH]
|Deer
[MESH]
|Diarrhea/prevention & control/*veterinary
[MESH]
|Disease Transmission, Infectious/*prevention & control
[MESH]
free
free
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