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10.1016/j.jsb.2016.06.001 http://scihub22266oqcxt.onion/10.1016/j.jsb.2016.06.001 C5464370!5464370 !27288865     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27288865 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Struct+Biol 2016 ; 195 (2 ): 190-198 Nephropedia Template TP {{tp|p=27288865 |t=2016. A new method for vitrifying samples for cryoEM |pdf=|usr=}}{{27288865 }} gab.com Text A new method for vitrifying samples for cryoEM JO: J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=27288865 #FOAvip Almost every aspect of cryo electron microscopy (cryoEM) has been automated over the last few decades. One of the challenges that remains to be addressed is the robust and reliable preparation of vitrified specimens of suitable ice thickness. We present results from a new device for preparing vitrified samples. The successful use of the device is coupled to a new "self-blotting" grid that we have developed to provide a method for spreading a sample to a thin film without the use of externally applied filter paper. This new approach has the advantage of using small amounts of protein material, resulting in large areas of ice of a well defined thickness containing evenly distributed single particles. We believe that these methods will in the future result in a system for vitrifying grids that is completely automated. Twit Text FOAVip A new method for vitrifying samples for cryoEM ????J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=27288865 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27288865 #FOAvip English Wikipedia <ref>{{Cite pmid|27288865 }}</ref> A new method for vitrifying samples for cryoEM #MMPMID27288865 Razinkov I ; Dandey V ; Wei H ; Zhang Z ; Melnekoff D ; Rice WJ ; Wigge C ; Potter CS ; Carragher B J Struct Biol 2016[Aug]; 195 (2 ): 190-198 PMID27288865 show ga Almost every aspect of cryo electron microscopy (cryoEM) has been automated over the last few decades. One of the challenges that remains to be addressed is the robust and reliable preparation of vitrified specimens of suitable ice thickness. We present results from a new device for preparing vitrified samples. The successful use of the device is coupled to a new "self-blotting" grid that we have developed to provide a method for spreading a sample to a thin film without the use of externally applied filter paper. This new approach has the advantage of using small amounts of protein material, resulting in large areas of ice of a well defined thickness containing evenly distributed single particles. We believe that these methods will in the future result in a system for vitrifying grids that is completely automated. |*Vitrification [MESH] |Cryoelectron Microscopy/*instrumentation/methods [MESH] |Ice/analysis [MESH] |Image Processing, Computer-Assisted [MESH]A new method for vitrifying samples for cryoEM (epmc).pdf DeepDyve Pubget Overpricing