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10.1371/journal.pone.0173914 http://scihub22266oqcxt.onion/10.1371/journal.pone.0173914 C5358814!5358814!28319158     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28319158.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2017 ; 12 (3): ä Nephropedia Template TP {{tp|p=28319158|t=2017. A morphological method for ammonia detection in liver |pdf=|usr=}}{{28319158}} gab.com Text A morphological method for ammonia detection in liver JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28319158 #FOAvip Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler´s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome. Twit Text FOAVip A morphological method for ammonia detection in liver ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28319158 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28319158 #FOAvip English Wikipedia <ref>{{Cite pmid|28319158}}</ref> A morphological method for ammonia detection in liver #MMPMID28319158 Gutiérrez-de-Juan V; López de Davalillo S; Fernández-Ramos D; Barbier-Torres L; Zubiete-Franco I; Fernández-Tussy P; Simon J; Lopitz-Otsoa F; de las Heras J; Iruzubieta P; Arias-Loste MT; Villa E; Crespo J; Andrade R; Lucena MI; Varela-Rey M; Lu SC; Mato JM; Delgado TC; Martínez-Chantar ML PLoS One 2017[]; 12 (3): ä PMID28319158show ga Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler´s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome. äA morphological method for ammonia detection in liver (epmc).pdf DeepDyve Pubget Overpricing