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2017 ; 12
(3
): e0173914
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A morphological method for ammonia detection in liver
#MMPMID28319158
Gutiérrez-de-Juan V
; López de Davalillo S
; Fernández-Ramos D
; Barbier-Torres L
; Zubiete-Franco I
; Fernández-Tussy P
; Simon J
; Lopitz-Otsoa F
; de Las Heras J
; Iruzubieta P
; Arias-Loste MT
; Villa E
; Crespo J
; Andrade R
; Lucena MI
; Varela-Rey M
; Lu SC
; Mato JM
; Delgado TC
; Martínez-Chantar ML
PLoS One
2017[]; 12
(3
): e0173914
PMID28319158
show ga
Hyperammonemia is a metabolic condition characterized by elevated levels of
ammonia and a common event in acute liver injury/failure and chronic liver
disease. Even though hepatic ammonia levels are potential predictive factors of
patient outcome, easy and inexpensive methods aiming at the detection of liver
ammonia accumulation in the clinical setting remain unavailable. Thus, herein we
have developed a morphological method, based on the utilization of Nessler´s
reagent, to accurately and precisely detect the accumulation of ammonia in
biological tissue. We have validated our method against a commercially available
kit in mouse tissue samples and, by using this modified method, we have confirmed
the hepatic accumulation of ammonia in clinical and animal models of acute and
chronic advanced liver injury as well as in the progression of fatty liver
disease. Overall, we propose a morphological method for ammonia detection in
liver that correlates well with the degree of liver disease severity and
therefore can be potentially used to predict patient outcome.
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