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2017 ; 45
(15
): 9085-9092
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A mechanism for a single nucleotide intron shift
#MMPMID28595329
Fekete E
; Flipphi M
; Ág N
; Kavalecz N
; Cerqueira G
; Scazzocchio C
; Karaffa L
Nucleic Acids Res
2017[Sep]; 45
(15
): 9085-9092
PMID28595329
show ga
Spliceosomal introns can occupy nearby rather than identical positions in
orthologous genes (intron sliding or shifting). Stwintrons are complex
intervening sequences, where an 'internal' intron interrupts one of the sequences
essential for splicing, generating after its excision, a newly formed canonical
intron defined as 'external'. In one experimentally demonstrated configuration,
two alternatively excised internal introns, overlapping by one G, disrupt
respectively the donor and the acceptor sequence of an external intron, leading
to mRNAs encoding identical proteins. In a gene encoding a DHA1 antiporter in
Pezizomycotina, we find a variety of predicted intron configurations interrupting
the DNA stretch encoding a conserved peptidic sequence. Some sport a stwintron
where the internal intron interrupts the donor of the external intron
(experimentally confirmed for Aspergillus nidulans). In others, we found and
demonstrate (for Trichoderma reesei) alternative, overlapping internal introns.
Discordant canonical introns, one nt apart, are present in yet other species,
exactly as predicted by the alternative loss of either of the internal introns at
the DNA level from an alternatively spliced stwintron. An evolutionary pathway of
1 nt intron shift, involving an alternatively spliced stwintron intermediate is
proposed on the basis of the experimental and genomic data presented.
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