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10.7554/eLife.22767 http://scihub22266oqcxt.onion/10.7554/eLife.22767 C5373822!5373822!28318487     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28318487.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       eLife 2017 ; 6 (ä): ä Nephropedia Template TP {{tp|p=28318487|t=2017. A high-resolution map of transcriptional repression |pdf=|usr=}}{{28318487}} gab.com Text A high-resolution map of transcriptional repression JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=28318487 #FOAvip Turning genes on and off is essential for development and homeostasis, yet little is known about the sequence and causal role of chromatin state changes during the repression of active genes. This is surprising, as defective gene silencing underlies developmental abnormalities and disease. Here we delineate the sequence and functional contribution of transcriptional repression mechanisms at high temporal resolution. Inducible entry of the NuRD-interacting transcriptional regulator Ikaros into mouse pre-B cell nuclei triggered immediate binding to target gene promoters. Rapid RNAP2 eviction, transcriptional shutdown, nucleosome invasion, and reduced transcriptional activator binding required chromatin remodeling by NuRD-associated Mi2beta/CHD4, but were independent of HDAC activity. Histone deacetylation occurred after transcriptional repression. Nevertheless, HDAC activity contributed to stable gene silencing. Hence, high resolution mapping of transcriptional repression reveals complex and interdependent mechanisms that underpin rapid transitions between transcriptional states, and elucidates the temporal order, functional role and mechanistic separation of NuRD-associated enzymatic activities.DOI:http://dx.doi.org/10.7554/eLife.22767.001 Twit Text FOAVip A high-resolution map of transcriptional repression ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=28318487 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28318487 #FOAvip English Wikipedia <ref>{{Cite pmid|28318487}}</ref> A high-resolution map of transcriptional repression #MMPMID28318487 Liang Z; Brown KE; Carroll T; Taylor B; Vidal IF; Hendrich B; Rueda D; Fisher AG; Merkenschlager M eLife 2017[]; 6 (ä): ä PMID28318487show ga Turning genes on and off is essential for development and homeostasis, yet little is known about the sequence and causal role of chromatin state changes during the repression of active genes. This is surprising, as defective gene silencing underlies developmental abnormalities and disease. Here we delineate the sequence and functional contribution of transcriptional repression mechanisms at high temporal resolution. Inducible entry of the NuRD-interacting transcriptional regulator Ikaros into mouse pre-B cell nuclei triggered immediate binding to target gene promoters. Rapid RNAP2 eviction, transcriptional shutdown, nucleosome invasion, and reduced transcriptional activator binding required chromatin remodeling by NuRD-associated Mi2beta/CHD4, but were independent of HDAC activity. Histone deacetylation occurred after transcriptional repression. Nevertheless, HDAC activity contributed to stable gene silencing. Hence, high resolution mapping of transcriptional repression reveals complex and interdependent mechanisms that underpin rapid transitions between transcriptional states, and elucidates the temporal order, functional role and mechanistic separation of NuRD-associated enzymatic activities.DOI:http://dx.doi.org/10.7554/eLife.22767.001 äA high-resolution map of transcriptional repression (epmc).pdf DeepDyve Pubget Overpricing