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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Am+Soc+Nephrol
2010 ; 21
(11
): 1835-41
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A high-powered view of the filtration barrier
#MMPMID20576805
Peti-Peterdi J
; Sipos A
J Am Soc Nephrol
2010[Nov]; 21
(11
): 1835-41
PMID20576805
show ga
Multiphoton excitation fluorescence microscopy is a powerful noninvasive imaging
technique for the deep optical sectioning of living tissues. Its application in
several intact tissues is a significant advance in our understanding of organ
function, including renal pathophysiological mechanisms. The glomerulus, the
filtering unit in the kidney, is one good example of a relatively inaccessible
and complex structure, with cell types that are otherwise difficult to study at
high resolution in their native environment. In this article, we address the
application, advantages, and limitations of this imaging technology for the study
of the glomerular filtration barrier and the controversy it recently generated
regarding the glomerular filtration of macromolecules. More advanced and accurate
multiphoton determinations of the glomerular sieving coefficient that are
presented here dismiss previous claims on the filtration of nephrotic levels of
albumin. The sieving coefficient of 70-kD dextran was found to be around 0.001.
Using a model of focal segmental glomerulosclerosis, increased filtration barrier
permeability is restricted only to areas of podocyte damage, consistent with the
generally accepted role of podocytes and the glomerular origin of albuminuria.
Time-lapse imaging provides new details and important in vivo confirmation of the
dynamics of podocyte movement, shedding, replacement, and the role of the
parietal epithelial cells and Bowman's capsule in the pathology of
glomerulosclerosis.
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