Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29309640
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nucleic+Acids+Res
2018 ; 46
(5
): 2495-2508
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
A fungal Argonaute interferes with RNA interference
#MMPMID29309640
Nguyen Q
; Iritani A
; Ohkita S
; Vu BV
; Yokoya K
; Matsubara A
; Ikeda KI
; Suzuki N
; Nakayashiki H
Nucleic Acids Res
2018[Mar]; 46
(5
): 2495-2508
PMID29309640
show ga
Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA
interference (RNAi), require a unique class of proteins called Argonautes (AGOs).
An AGO protein typically forms a protein-sRNA complex that contributes to gene
silencing using the loaded sRNA as a specificity determinant. Here, we show that
MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe
oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and
MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon
(MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (?moago2)
showed elevated levels of gene silencing. Consistently, transcript levels of
MAGGY and mycoviruses were drastically reduced in ?moago2, supporting the idea
that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis
revealed that repeat- and mycovirus-derived small interfering RNAs were mainly
associated with MoAGO2 and MoAGO3, and their populations were very similar based
on their size distribution patterns and positional base preference. Site-directed
mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2
was essential for the ability to diminish the efficacy of RNAi. Overall, these
results suggest a possible interplay between distinct sRNA-mediated gene
regulation pathways through a competition for sRNA.
free
free
free
