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10.1093/nar/gkw108 http://scihub22266oqcxt.onion/10.1093/nar/gkw108 C4889923!4889923 !26951376     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26951376 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acids+Res 2016 ; 44 (10 ): e92 Nephropedia Template TP {{tp|p=26951376 |t=2016. A conformation-induced fluorescence method for microRNA detection |pdf=|usr=}}{{26951376 }} gab.com Text A conformation-induced fluorescence method for microRNA detection JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26951376 #FOAvip MicroRNAs play important roles in a large variety of biological systems and processes through their regulation of target mRNA expression, and show promise as clinical biomarkers. However, their small size presents challenges for tagging or direct detection. Innovation in techniques to sense and quantify microRNAs may aid research into novel aspects of microRNA biology and contribute to the development of diagnostics. By introducing an additional stem loop into the fluorescent RNA Spinach and altering its 3' and 5' ends, we have generated a new RNA, Pandan, that functions as the basis for a microRNA sensor. Pandan contains two sequence-variable stem loops that encode complementary sequence for a target microRNA of interest. In its sensor form, it requires the binding of a target microRNA in order to reconstitute the RNA scaffold for fluorophore binding and fluorescence. Binding of the target microRNA resulted in large changes in fluorescence intensity. The median fold change in fluorescence observed for the sensors tested was ?50-fold. Pandan RNA sensors exhibit good signal-to-noise ratios, and can detect their target microRNAs within complex RNA mixtures. Twit Text FOAVip A conformation-induced fluorescence method for microRNA detection ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26951376 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26951376 #FOAvip English Wikipedia <ref>{{Cite pmid|26951376 }}</ref> A conformation-induced fluorescence method for microRNA detection #MMPMID26951376 Aw SS ; Tang MX ; Teo YN ; Cohen SM Nucleic Acids Res 2016[Jun]; 44 (10 ): e92 PMID26951376 show ga MicroRNAs play important roles in a large variety of biological systems and processes through their regulation of target mRNA expression, and show promise as clinical biomarkers. However, their small size presents challenges for tagging or direct detection. Innovation in techniques to sense and quantify microRNAs may aid research into novel aspects of microRNA biology and contribute to the development of diagnostics. By introducing an additional stem loop into the fluorescent RNA Spinach and altering its 3' and 5' ends, we have generated a new RNA, Pandan, that functions as the basis for a microRNA sensor. Pandan contains two sequence-variable stem loops that encode complementary sequence for a target microRNA of interest. In its sensor form, it requires the binding of a target microRNA in order to reconstitute the RNA scaffold for fluorophore binding and fluorescence. Binding of the target microRNA resulted in large changes in fluorescence intensity. The median fold change in fluorescence observed for the sensors tested was ?50-fold. Pandan RNA sensors exhibit good signal-to-noise ratios, and can detect their target microRNAs within complex RNA mixtures. |Aptamers, Nucleotide/*chemistry [MESH] |Benzyl Compounds/metabolism [MESH] |Biosensing Techniques/*methods [MESH] |Fluorescence [MESH] |Fluorescent Dyes [MESH] |Imidazolines/metabolism [MESH] |MicroRNAs/*analysis/chemistry [MESH]A conformation-induced fluorescence method for microRNA detection (epmc).pdf DeepDyve Pubget Overpricing