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2016 ; 17
(ä): 74
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A benchmark for RNA-seq quantification pipelines
#MMPMID27107712
Teng M
; Love MI
; Davis CA
; Djebali S
; Dobin A
; Graveley BR
; Li S
; Mason CE
; Olson S
; Pervouchine D
; Sloan CA
; Wei X
; Zhan L
; Irizarry RA
Genome Biol
2016[Apr]; 17
(ä): 74
PMID27107712
show ga
Obtaining RNA-seq measurements involves a complex data analytical process with a
large number of competing algorithms as options. There is much debate about which
of these methods provides the best approach. Unfortunately, it is currently
difficult to evaluate their performance due in part to a lack of sensitive
assessment metrics. We present a series of statistical summaries and plots to
evaluate the performance in terms of specificity and sensitivity, available as a
R/Bioconductor package ( http://bioconductor.org/packages/rnaseqcomp ). Using two
independent datasets, we assessed seven competing pipelines. Performance was
generally poor, with two methods clearly underperforming and RSEM slightly
outperforming the rest.