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10.1038/srep15342 http://scihub22266oqcxt.onion/10.1038/srep15342 C4615978!4615978 !26493176     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26493176 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 15342 Nephropedia Template TP {{tp|p=26493176 |t=2015. A Versatile Microarray Platform for Capturing Rare Cells |pdf=|usr=}}{{26493176 }} gab.com Text A Versatile Microarray Platform for Capturing Rare Cells JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26493176 #FOAvip Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs)--about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences. Twit Text FOAVip A Versatile Microarray Platform for Capturing Rare Cells ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26493176 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26493176 #FOAvip English Wikipedia <ref>{{Cite pmid|26493176 }}</ref> A Versatile Microarray Platform for Capturing Rare Cells #MMPMID26493176 Brinkmann F ; Hirtz M ; Haller A ; Gorges TM ; Vellekoop MJ ; Riethdorf S ; Müller V ; Pantel K ; Fuchs H Sci Rep 2015[Oct]; 5 (ä): 15342 PMID26493176 show ga Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs)--about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences. |*Lab-On-A-Chip Devices [MESH] |*Oligonucleotide Array Sequence Analysis [MESH] |Humans [MESH]A Versatile Microarray Platform for Capturing Rare Cells (epmc).pdf DeepDyve Pubget Overpricing