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2015 ; 55
(6-7
): 698-710
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A Newcomer s Guide to Peptide Crystallography
#MMPMID26213415
Spencer RK
; Nowick JS
Isr J Chem
2015[Jun]; 55
(6-7
): 698-710
PMID26213415
show ga
Here we provide a guide for adapting the tools developed for protein X-ray
crystallography to study the structures and supramolecular assembly of peptides.
Peptide crystallography involves selecting a suitable peptide, crystallizing the
peptide, collecting X-ray diffraction data, processing the diffraction data,
determining the crystallographic phases and generating an electron density map,
building and refining models, and depositing the crystallographic structure in
the Protein Data Bank (PDB). Advances in technology make this process easy for a
newcomer to adopt. This paper describes techniques for determining the X-ray
crystallographic structures of peptides: incorporation of amino acids containing
heavy atoms for crystallographic phase determination, commercially available kits
to crystallize peptides, modern techniques for X-ray crystallographic data
collection, and free user-friendly software for data processing and producing a
crystallographic structure.