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2017 ; 90
(1
): 87-95
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A Cell-Based Assay to Assess Hemichannel Function
#MMPMID28356896
Krishnan S
; Fiori MC
; Cuello LG
; Altenberg GA
Yale J Biol Med
2017[Mar]; 90
(1
): 87-95
PMID28356896
show ga
Activation of connexin hemichannels is involved in the pathophysiology of
disorders that include deafness, stroke, and cardiac infarct. This aspect makes
hemichannels an attractive therapeutic target. Unfortunately, most available
inhibitors are not selective or isoform specific, which hampers their
translational application. The absence of a battery of useful inhibitors is due
in part to the absence of simple screening assays for the discovery of
hemichannel-active drugs. Here, we present an assay that we have recently
developed to assess hemichannel function. The assay is based on the expression of
functional human connexins in a genetically modified bacterial strain deficient
in K(+) uptake. These modified cells do not grow in low-K(+) medium, but
functional expression of connexin hemichannels allows K(+) uptake and growth.
This cell-growth-based assay is simple, robust, and easily scalable to
high-throughput multi-well platforms.
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