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Brimmo A
; Goyette PA
; Alnemari R
; Gervais T
; Qasaimeh MA
Sci Rep
2018[Jul]; 8
(1
): 10995
PMID30030464
show ga
In this work, we fabricate microfluidic probes (MFPs) in a single step by
stereolithographic 3D printing and benchmark their performance with standard MFPs
fabricated via glass or silicon micromachining. Two research teams join forces to
introduce two independent designs and fabrication protocols, using different
equipment. Both strategies adopted are inexpensive and simple (they only require
a stereolithography printer) and are highly customizable. Flow characterization
is performed by reproducing previously published microfluidic dipolar and
microfluidic quadrupolar reagent delivery profiles which are compared to the
expected results from numerical simulations and scaling laws. Results show that,
for most MFP applications, printer resolution artifacts have negligible impact on
probe operation, reagent pattern formation, and cell staining results. Thus, any
research group with a moderate resolution (?100?µm) stereolithography printer
will be able to fabricate the MFPs and use them for processing cells, or
generating microfluidic concentration gradients. MFP fabrication involved glass
and/or silicon micromachining, or polymer micromolding, in every previously
published article on the topic. We therefore believe that 3D printed MFPs is
poised to democratize this technology. We contribute to initiate this trend by
making our CAD files available for the readers to test our "print & probe"
approach using their own stereolithographic 3D printers.
free
free
free
