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Methods of examining induced sputum: do differences matter?Kips JC; Peleman RA; Pauwels RAEur Respir J 1998[Mar]; 11 (3): 529-33Analysis of induced sputum has been proposed as a direct, relatively noninvasive method for the evaluation of airway inflammation in diseases such as asthma or chronic obstructive pulmonary disease (COPD). An important question in the validation of this technique concerns the potential influence of differences in the methods of examining sputum. Up to the present time, two basic techniques for processing sputum have been described. The first approach consists of selecting all viscid portions from the expectorated sample, whereas the second approach processes the whole expectorate, containing sputum plus saliva. Both processing techniques have been shown to provide valid and reliable data on the composition of the cellular and soluble fraction of induced sputum. From the data currently available, it would therefore appear that the usefulness of induced sputum as a method for assessing airway inflammation is not influenced by differences in the methods currently used for examining sputum.|Humans[MESH]|Saliva[MESH]|Specimen Handling/methods[MESH]|Sputum/*chemistry/metabolism[MESH] |
