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lüll Interactions of the borna disease virus P, N, and X proteins and their functional implications Schwemmle M; Salvatore M; Shi L; Richt J; Lee CH; Lipkin WIJ Biol Chem 1998[Apr]; 273 (15): 9007-12Borna disease virus (BDV) causes persistent central nervous system infection and behavioral disturbances in warm-blooded animals. Protein interaction studies were pursued to gain insight into the functions of the putative nucleoprotein (N), phosphoprotein (P), atypical glycoprotein (gp18), and X protein (X) of BDV. Coimmunoprecipitation experiments indicated that N and P, and P and X, form complexes in infected cells. Two-hybrid analyses confirmed interactions between P and P, P and X, and P and N, but not between P and gp18, N and gp18, X and gp18, or X and N. Analysis of P truncation mutants identified three nonoverlapping regions important for oligomerization (amino acids (aa) 135-172), and binding to X (aa 33-115) or N (aa 197-201). Coexpression of X stimulated oligomerization of P but decreased N-P complex formation. Immunocytochemistry of transfected noninfected CHO cells demonstrated that the distribution of X is dependent upon the presence of P-X expressed alone was found predominantly in the cytoplasm whereas coexpression of X and P resulted in nuclear localization. Immunocytochemistry of infected cells revealed nuclear colocalization of P and X. Interactions of P, N, and X may have implications for regulation of BDV transcription/replication and ribonucleoprotein assembly.|*Saccharomyces cerevisiae Proteins[MESH]|*Transcription Factors[MESH]|Animals[MESH]|Borna disease virus/genetics/*physiology[MESH]|CHO Cells[MESH]|COS Cells[MESH]|Cell Line[MESH]|Cell Nucleus/metabolism/ultrastructure[MESH]|Cricetinae[MESH]|Cysteine/metabolism[MESH]|DNA-Binding Proteins[MESH]|Fungal Proteins/biosynthesis[MESH]|Macromolecular Substances[MESH]|Methionine/metabolism[MESH]|Open Reading Frames[MESH]|Protein Biosynthesis[MESH]|Protein Multimerization[MESH]|Recombinant Fusion Proteins/biosynthesis[MESH]|Transfection[MESH]|Two-Hybrid System Techniques[MESH]|Viral Proteins/isolation & purification/*metabolism[MESH] |