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Rapid validation of molecular structures of biological samples by electrospray-mass spectrometry Ashton DS; Beddell CR; Green BN; Oliver RWFEBS Lett 1994[Mar]; 342 (1): 1-6A short account is presented of the method of measuring molecular masses (M(r)) of pure biological samples by electrospray ionisation mass spectrometry. It is demonstrated that the technique yields M(r) values with an effective accuracy equal to or better than 0.008% of the calculated M(r), provided that the correct molecular structure is employed in the calculation. It is therefore recommended that this method of measuring M(r)'s should be considered to form an essential part of all studies aimed at elucidating the molecular structure of purified biological macromolecules or for confirming the identity of labelled samples of such molecules.|Animals[MESH]|Humans[MESH]|Mass Spectrometry/*methods[MESH]|Molecular Structure[MESH]|Molecular Weight[MESH]|Protein Conformation[MESH]|Proteins/*chemistry[MESH] |