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lüll COPII: a membrane coat that forms endoplasmic reticulum-derived vesicles Barlowe CFEBS Lett 1995[Aug]; 369 (1): 93-6Vesicle budding from the endoplasmic reticulum (ER) has been reconstituted with washed membranes and three soluble proteins: Sec13 complex, Sec23 complex and the small GTPase Sar1p. The proteins that drive this cell-free vesicle budding reaction form an approximately 10 nm thick electron dense coat on ER-derived vesicles. Although the overall mechanism of membrane budding driven by various cytoplasmic coats appears similar, the constituents of this new membrane coat are molecularly distinct from the non-clathrin coat (COP) involved in intra-Golgi transport and the clathrin-containing coats. The new vesicle coat has been termed COPII.|*Monomeric GTP-Binding Proteins[MESH]|*Saccharomyces cerevisiae Proteins[MESH]|Biological Transport[MESH]|COP-Coated Vesicles[MESH]|Endoplasmic Reticulum/*metabolism[MESH]|Fungal Proteins/*metabolism[MESH]|GTP-Binding Proteins/*metabolism[MESH]|GTPase-Activating Proteins[MESH]|Intracellular Membranes/*metabolism[MESH]|Membrane Proteins/*metabolism[MESH]|Nuclear Pore Complex Proteins[MESH]|Vesicular Transport Proteins[MESH] |