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lüll Alternative hypotheses of proton ejection in cytochrome oxidase vesicles Transmembrane proton pumping or redox-linked deprotonation of phospholipid-cytochrome c complex(es) Mitchell P; Moyle JFEBS Lett 1983[Jan]; 151 (2): 167-78A review of published experimental and interpretative knowledge concerning proton ejection associated with cytochrome c oxidation by artificial phospholipid vesicles inlaid with cytochrome c oxidase indicates that the detailed characteristics of the redox-linked proton ejection cannot be simply explained by proton pumping. We propose an alternative hypothesis according to which proton ejection is due to the redox-linked deprotonation of a complex involving phospholipid and cytochrome c at the surface of the vesicles. The postulates upon which this hypothesis depends are explicitly outlined, and some methods of testing the hypothesis are suggested.|Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology[MESH]|Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology[MESH]|Cytochrome c Group/*metabolism[MESH]|Dicyclohexylcarbodiimide/pharmacology[MESH]|Electron Transport[MESH]|Electron Transport Complex IV/*metabolism[MESH]|Hydrogen-Ion Concentration[MESH]|Kinetics[MESH]|Liposomes[MESH]|Oxidation-Reduction[MESH]|Phospholipids/*pharmacology[MESH] |