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lüll Lanthanum as an electron microscopic stain Shaklai M; Tavassoli MJ Histochem Cytochem 1982[Dec]; 30 (12): 1325-30Applications of lanthanum as an electron microscopic tracer have been reviewed. This electron-dense trivalent cation, which binds avidly to calcium binding sites, can be used as tracer for delineating extracellular spaces and intercellular junctions. It has served as a basis for classification of junctional structures. It can also be used as a calcium probe, a tracer in studying the permeability of barriers, as an intracellular marker and as an electron microscopic stain for such membrane components as surface glycoprotein. Each of these applications may require a different methodology. Thus methodological considerations in the use of this tracer have also been reviewed. The recent recognition that lanthanum is more than a passive tracer and that by reacting with different cell components may serve as a true stain, will extend the use of lanthanum in electron microscope histochemistry.|*Histocytochemistry[MESH]|*Lanthanum[MESH]|*Microscopy, Electron[MESH]|*Staining and Labeling[MESH]|Animals[MESH]|Calcium-Binding Proteins/analysis[MESH]|Cell Membrane Permeability[MESH]|Cell Membrane/analysis[MESH]|Chemical Precipitation[MESH]|Colloids[MESH]|Extracellular Space[MESH]|Freeze Fracturing[MESH]|Intercellular Junctions/ultrastructure[MESH]|Intracellular Membranes/analysis[MESH]|Organoids/analysis/ultrastructure[MESH]|Permeability[MESH]|Phospholipids/analysis[MESH] |