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Ascites production in 9 rat strains Douglas EG; Hamada Y; McKearn TJJ Immunol Methods 1979[]; 26 (1): 69-74A simple inexpensive, and rapid method for inducing large volumes of ascitic fluid in rats is described. The procedure involves intraperitoneal injections of Freund's adjuvant emulsion on alternate days. Antibody activity of the ascitic fluid approximates that found in the serum. Female rats consistently respond better than males. Members of all 9 strains of rats tested produced ascites using this procedure.|Animals[MESH]|Antibodies[MESH]|Ascites/*immunology[MESH]|Erythrocytes/immunology[MESH]|Female[MESH]|Immunization[MESH]|Injections, Intraperitoneal[MESH]|Male[MESH]|Rats[MESH]|Rats, Inbred ACI[MESH]|Rats, Inbred BN[MESH]|Rats, Inbred Lew[MESH]|Sheep[MESH] |
