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Antigen detection for human immunodeficiency virus Harry DJ; Jennings MB; Yee J; Carlson JRClin Microbiol Rev 1989[Jul]; 2 (3): 241-9The recent development of enzyme immunoassay procedures for the direct determination of human immunodeficiency virus (HIV) antigens has been of significant benefit in both clinical and research applications. The historical development of HIV antigen assays as well as their current and future applications for use in the clinical microbiology laboratory are reviewed. A detailed description of selected commercially available assays is presented, and a comparison is made of various parameters, including sensitivity, specificity, and cost. The use of the HIV antigen assay as an alternative to the reverse transcriptase assay in virus culture applications is also discussed. In addition, the diagnostic and prognostic utility of the HIV antigen assay is considered for various patient groups, including neonatal, high-risk asymptomatic, seronegative, and seropositive patient populations. The use of the HIV antigen assay as an adjunct to anti-HIV antibody testing, as well as its utility in assessing the therapeutic efficacy of antiviral drug therapy, is discussed. The biology of HIV antigen expression and modulation of anti-HIV antibody titers during infection are also discussed in terms of two possible models.|AIDS Serodiagnosis/methods[MESH]|Acquired Immunodeficiency Syndrome/*diagnosis[MESH]|HIV Antigens/*analysis[MESH]|HIV/*immunology[MESH]|Humans[MESH]|Infant[MESH]|Reagent Kits, Diagnostic[MESH]|Virus Cultivation[MESH] |
