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lüll Antimicrobial Disk Susceptibility Testing of Leptospira spp Using Leptospira Vanaporn Wuthiekanun (LVW) Agar Wuthiekanun V; Amornchai P; Langla S; White NJ; Day NPJ; Limmathurotsakul D; Peacock SJAm J Trop Med Hyg 2015[Aug]; 93 (2): 241-243Leptospira Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for Leptospira spp. Ten pathogenic Leptospira isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin/clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, nitrofurantoin, penicillin, piperacillin/tazobactam, and tetracycline). All 10 isolates had no zone of growth inhibition for four antimicrobials (fosfomycin, nalidixic acid, rifampicin, and trimethoprim/sulfamethoxazole). Of the ten Leptospira, seven had a growth inhibition zone of = 21 mm for aztreonam, the zone diameter susceptibility break point for Enterobacteriaceae. This assay could find utility as a simple screening method during the epidemiological surveillance of antimicrobial resistance in Leptospira spp.|*Drug Resistance, Multiple, Bacterial[MESH]|Anti-Bacterial Agents/*pharmacology[MESH]|Ciprofloxacin/pharmacology[MESH]|Gentamicins/pharmacology[MESH]|Leptospira/*drug effects[MESH]|Microbial Sensitivity Tests/methods[MESH]|Penicillanic Acid/analogs & derivatives/pharmacology[MESH]|Piperacillin, Tazobactam Drug Combination[MESH]|Piperacillin/pharmacology[MESH]|Rifampin/pharmacology[MESH]|Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology[MESH] |