Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
free
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
free free
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Hepatopulmonary syndrome: the role of intra-abdominal hypertension and a novel mouse model Zhang Z; Qi X; Li Z; Xu L; Wang F; Wang S; Chang Y; Ma W; Xu M; Yang CInt J Clin Exp Pathol 2014[]; 7 (2): 768-73OBJECTIVE: Hepatopulmonary syndrome (HPS) is considered as a triad of chronic liver disease, pulmonary vascular ectasia and severe hypoxemia. The study aims to investigate the pathological mechanism of intra-abdominal pressure (IAP) in HPS and establish a novel mouse model. METHODS: Fifty male ICR mice were randomly divided into experimental and control group, receiving subcutaneous injection of carbon tetrachloride and water, respectively. Mice in experimental group were then divided into 4 sub-groups with the intraperitoneal injection of different volume of albumin to form different IAP (0, 5, 10 and 20 cmH2O). All the mice were then sacrificed 24 hours later and blood gas analysis was conducted. In addition, liver and lung histopathology was also examined. RESULTS: Blood gas analysis in different IAP suggested the respiratory alkalosis. Arterial partial pressure of oxygen significantly decreased in the IAP=10 cmH2O (68.13 +/- 3.56, P<0.01) and 20 cmH2O (66.00 +/- 3.78, P<0.01). Alveolar-arterial oxygen pressure difference increased markedly in the IAP=10 cmH2O (54.60 +/- 6.80, P<0.001) and 20 cmH2O (57.04 +/- 5.60, P<0.001). According to lung histopathology, macrophages were found to accumulate in the alveolar spaces and the widened alveolar walls were detected. In addition, there was visible blood stasis in the alveolar walls and numerous red blood cells extravasated into air space in the IAP=10 and 20 cmH2O. CONCLUSIONS: Our study suggested that intra-abdominal hypertension was a significant pathological mechanism of HPS. Meanwhile, we have established a novel mouse model that will now be optimized with further investigation of the mechanism and therapeutic targets of HPS.|Albumins[MESH]|Alkalosis, Respiratory/blood/etiology[MESH]|Animals[MESH]|Blood Gas Analysis[MESH]|Carbon Tetrachloride[MESH]|Hepatopulmonary Syndrome/blood/*etiology/pathology[MESH]|Intra-Abdominal Hypertension/blood/chemically induced/*complications/pathology[MESH]|Liver Cirrhosis, Experimental/blood/chemically induced/*complications/pathology[MESH]|Liver/pathology[MESH]|Macrophages/pathology[MESH]|Male[MESH]|Mice[MESH]|Mice, Inbred ICR[MESH]|Oxygen/blood[MESH]|Partial Pressure[MESH]|Pulmonary Alveoli/metabolism/pathology[MESH]|Time Factors[MESH] |