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lüll Pericytes from infantile hemangioma display proangiogenic properties and dysregulated angiopoietin-1 Boscolo E; Mulliken JB; Bischoff JArterioscler Thromb Vasc Biol 2013[Mar]; 33 (3): 501-9OBJECTIVE: Infantile hemangioma (IH) is a rapidly growing vascular tumor affecting newborns. It is composed of immature endothelial cells and pericytes that proliferate into a disorganized mass of blood vessels. We isolated pericytes from IH (Hem-pericytes) to test our hypothesis that Hem-pericytes are unable to stabilize blood vessels. METHODS AND RESULTS: We injected pericytes in vivo, in combination with endothelial cells, and found that Hem-pericytes formed more microvessels compared with control retinal pericytes. We, thereby, analyzed proangiogenic properties of the Hem-pericytes. They grew fast in vitro, and were unable to stabilize endothelial cell growth and migration, and expressed high levels of vascular endothelial growth factor-A compared with retinal pericytes. Hem-pericytes from proliferating phase IH showed lower contractility in vitro, compared with Hem-pericytes from the involuting phase and retinal pericytes. Consistent with a diminished ability to stabilize endothelium, angiopoietin 1 was reduced in Hem-pericytes compared with retinal pericytes. Normal retinal pericytes in which angiopoietin 1 was silenced produced conditioned medium that stimulated endothelial cell proliferation and migration. CONCLUSIONS: We report the first successful isolation of patient-derived pericytes from IH tissue. Hem-pericytes exhibited proangiogenic properties and low levels of angiopoietin 1, consistent with a diminished ability to stabilize blood vessels in IH.|*Neovascularization, Pathologic[MESH]|Angiopoietin-1/genetics/*metabolism[MESH]|Animals[MESH]|Biomarkers/metabolism[MESH]|Cell Communication[MESH]|Cell Movement[MESH]|Cell Proliferation[MESH]|Cell Separation/methods[MESH]|Cells, Cultured[MESH]|Culture Media, Conditioned/metabolism[MESH]|Down-Regulation[MESH]|Hemangioma/*metabolism/pathology[MESH]|Human Umbilical Vein Endothelial Cells/metabolism/transplantation[MESH]|Humans[MESH]|Infant[MESH]|Infant, Newborn[MESH]|Male[MESH]|Mice[MESH]|Mice, Nude[MESH]|Pericytes/*metabolism/pathology/transplantation[MESH]|RNA Interference[MESH]|RNA, Messenger/metabolism[MESH]|Retinal Vessels/metabolism[MESH]|Time Factors[MESH]|Transfection[MESH]|Vascular Endothelial Growth Factor A/metabolism[MESH] |