suck pdf from google scholar
PDF vom PMID22275752 :   free
suck pdf from library genesis
English Wikipedia

Nephropedia Template TP (

Twit Text


  • DeepDyve
  • Pubget Overpricing


  • lüll
  • Fibroblast growth factor-23 abolishes 1,25-dihydroxyvitamin D(3)-enhanced duodenal calcium transport in male mice
  • Khuituan P; Teerapornpuntakit J; Wongdee K; Suntornsaratoon P; Konthapakdee N; Sangsaksri J; Sripong C; Krishnamra N; Charoenphandhu N
  • Am J Physiol Endocrinol Metab 2012[Apr]; 302 (8): E903-13
  • Despite being widely recognized as the important bone-derived phosphaturic hormone, whether fibroblast growth factor (FGF)-23 modulated intestinal calcium absorption remained elusive. Since FGF-23 could reduce the circulating level of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)], FGF-23 probably compromised the 1,25(OH)(2)D(3)-induced intestinal calcium absorption. FGF-23 may also exert an inhibitory action directly through FGF receptors (FGFR) in the intestinal cells. Herein, we demonstrated by Ussing chamber technique that male mice administered 1 mug/kg 1,25(OH)(2)D(3) sc daily for 3 days exhibited increased duodenal calcium absorption, which was abolished by concurrent intravenous injection of recombinant mouse FGF-23. This FGF-23 administration had no effect on the background epithelial electrical properties, i.e., short-circuit current, transepithelial potential difference, and resistance. Immunohistochemical evidence of protein expressions of FGFR isoforms 1-4 in mouse duodenal epithelial cells suggested a possible direct effect of FGF-23 on the intestine. This was supported by the findings that FGF-23 directly added to the serosal compartment of the Ussing chamber and completely abolished the 1,25(OH)(2)D(3)-induced calcium absorption in the duodenal tissues taken from the 1,25(OH)(2)D(3)-treated mice. However, direct FGF-23 exposure did not decrease the duodenal calcium absorption without 1,25(OH)(2)D(3) preinjection. The observed FGF-23 action was mediated by MAPK/ERK, p38 MAPK, and PKC. Quantitative real-time PCR further showed that FGF-23 diminished the 1,25(OH)(2)D(3)-induced upregulation of TRPV5, TRPV6, and calbindin-D(9k), but not PMCA(1b) expression in the duodenal epithelial cells. In conclusion, besides being a phosphatonin, FGF-23 was shown to be a novel calcium-regulating hormone that acted directly on the mouse intestine, thereby compromising the 1,25(OH)(2)D(3)-induced calcium absorption.
  • |*Intestinal Absorption/drug effects[MESH]
  • |Animals[MESH]
  • |Calbindins[MESH]
  • |Calcitriol/*metabolism[MESH]
  • |Calcium Channels/genetics/metabolism[MESH]
  • |Calcium, Dietary/*metabolism[MESH]
  • |Cell Polarity[MESH]
  • |Duodenum/cytology/drug effects/*metabolism[MESH]
  • |Fibroblast Growth Factors/genetics/*metabolism[MESH]
  • |Gene Expression Regulation[MESH]
  • |In Vitro Techniques[MESH]
  • |Intestinal Mucosa/cytology/drug effects/*metabolism[MESH]
  • |MAP Kinase Signaling System/drug effects[MESH]
  • |Male[MESH]
  • |Mice[MESH]
  • |Mice, Inbred ICR[MESH]
  • |Organ Specificity[MESH]
  • |Protein Isoforms/genetics/metabolism[MESH]
  • |Protein Kinase Inhibitors/pharmacology[MESH]
  • |RNA, Messenger/metabolism[MESH]
  • |Receptors, Fibroblast Growth Factor/antagonists & inhibitors/metabolism[MESH]
  • |Recombinant Proteins/metabolism[MESH]
  • |S100 Calcium Binding Protein G/genetics/metabolism[MESH]
  • |TRPV Cation Channels/genetics/metabolism[MESH]





  • *{{pmid22275752}}
    *<b>[http://www.kidney.de/mlpefetch.php?search=22275752 Fibroblast growth factor-23 abolishes 1,25-dihydroxyvitamin D(3)-enhanced duodenal calcium transport in male mice ]</b> Am J Physiol Endocrinol Metab 2012; 302(8) ; E903-13 Khuituan P; Teerapornpuntakit J; Wongdee K; Suntornsaratoon P; Konthapakdee N; Sangsaksri J; Sripong C; Krishnamra N; Charoenphandhu N

        *22275752*

    Nephropedia PMID record

    Deutsche Wikipedia - im Artikel

    Hier den unten stehenden Textblock hineinkopieren

    Am J Physiol Endocrinol Metab

    E903 8.302 2012