Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
free
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
free free
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Hypotonicity-induced TRPV4 function in renal collecting duct cells: modulation by progressive cross-talk with Ca2+-activated K+ channels Jin M; Berrout J; Chen L; O'Neil RGCell Calcium 2012[Feb]; 51 (2): 131-9The mouse cortical collecting duct (CCD) M-1 cells were grown to confluency on coverslips to assess the interaction between TRPV4 and Ca(2+)-activated K(+) channels. Immunocytochemistry demonstrated strong expression of TRPV4, along with the CCD marker, aquaporin-2, and the Ca(2+)-activated K(+) channels, the small conductance SK3 (K(Ca)2.3) channel and large conductance BKalpha channel (K(Ca)1.1). TRPV4 overexpression studies demonstrated little physical dependency of the K(+) channels on TRPV4. However, activation of TRPV4 by hypotonic swelling (or GSK1016790A, a selective agonist) or inhibition by the selective antagonist, HC-067047, demonstrated a strong dependency of SK3 and BK-alpha activation on TRPV4-mediated Ca(2+) influx. Selective inhibition of BK-alpha channel (Iberiotoxin) or SK3 channel (apamin), thereby depolarizing the cells, further revealed a significant dependency of TRPV4-mediated Ca(2+) influx on activation of both K(+) channels. It is concluded that a synergistic cross-talk exists between the TRPV4 channel and SK3 and BK-alpha channels to provide a tight functional regulation between the channel groups. This cross-talk may be progressive in nature where the initial TRPV4-mediated Ca(2+) influx would first activate the highly Ca(2+)-sensitive SK3 channel which, in turn, would lead to enhanced Ca(2+) influx and activation of the less Ca(2+)-sensitive BK channel.|Animals[MESH]|Apamin/pharmacology[MESH]|Aquaporin 2/biosynthesis[MESH]|Calcium/*metabolism[MESH]|Cell Line[MESH]|Gene Expression Regulation/drug effects/physiology[MESH]|Kidney Tubules, Collecting/cytology/*metabolism[MESH]|Large-Conductance Calcium-Activated Potassium Channels/agonists/antagonists & inhibitors/*metabolism[MESH]|Leucine/analogs & derivatives/pharmacology[MESH]|Mice[MESH]|Peptides/pharmacology[MESH]|Small-Conductance Calcium-Activated Potassium Channels/agonists/antagonists & inhibitors/*metabolism[MESH]|Sulfonamides/pharmacology[MESH]|TRPV Cation Channels/*metabolism[MESH] |