Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Tunicamycin negatively regulates BMP2-induced osteoblast differentiation through CREBH expression in MC3T3E1 cells Jang WG; Kim EJ; Koh JTBMB Rep 2011[Nov]; 44 (11): 735-40Tunicamycin, an endoplasmic reticulum (ER) stress inducer, specifically inhibits N-glycosylation. The cyclic AMP (cAMP) response element-binding protein H (CREBH) was previously shown to be regulated by UPR-dependent proteolytic cleavage in the liver. On the other hand, the role of CREBH in other tissues is unknown. In the present study, tunicamycin increased the level of CREBH activation (cleavage) as well as mRNA expression in osteoblast cells. Adenoviral (Ad) overexpression of CREBH suppressed BMP2-induced expression of alkaline phosphatase (ALP) and osteocalcin (OC). Interestingly, the BMP2-induced OASIS (structurally similar to CREBH, a positive regulator of osteoblast differentiation) expression was also inhibited by CREBH overexpression. In addition, inhibition of CREBH expression using siRNA reversed the tunicamycin-suppressed ALP and OC expression. These results suggest that CREBH inhibited osteoblast differentiation via suppressing BMP2-induced ALP, OC and OASIS expression in mouse calvarial derived osteoblasts.|Alkaline Phosphatase/metabolism[MESH]|Animals[MESH]|Bone Morphogenetic Protein 2/*pharmacology[MESH]|Cell Differentiation/*drug effects/genetics[MESH]|Cell Line[MESH]|Cyclic AMP Response Element-Binding Protein/genetics/*metabolism[MESH]|Endoplasmic Reticulum Stress/drug effects[MESH]|Gene Expression Regulation/drug effects[MESH]|Humans[MESH]|Mice[MESH]|Nerve Tissue Proteins/metabolism[MESH]|Osteoblasts/*cytology/*drug effects/enzymology[MESH]|Osteocalcin/metabolism[MESH]|Osteogenesis/drug effects/genetics[MESH]|Tunicamycin/*pharmacology[MESH] |