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An electrochemical functional assay for the sensing of nitric oxide release induced by angiogenic factors Trouillon R; O'Hare D; Chang SIBMB Rep 2011[Nov]; 44 (11): 699-704Nitric oxide (NO) is a critical biological mediator involved in numerous diseases. However, the short lifetime of this molecule in biological conditions can make its study in situ complicated. Here, we review some recent results on the role of NO in angiogenesis, obtained using a biocompatible microelectrode array. This simple system allowed for the quick and easy quantification of NO released from cells grown directly on the surface of the sensor. We have used this technology to demonstrate that angiogenin induces NO release, and to partially elucidate its intracellular transduction pathway.|Angiogenesis Inducing Agents/*metabolism[MESH]|Animals[MESH]|Biological Assay/*methods[MESH]|Electrochemical Techniques/*methods[MESH]|Humans[MESH]|Microelectrodes[MESH]|Models, Biological[MESH]|Nitric Oxide/*metabolism[MESH] |
