Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Effects of TRPV4 cation channel activation on the primary bladder afferent activities of the rat Aizawa N; Wyndaele JJ; Homma Y; Igawa YNeurourol Urodyn 2012[Jan]; 31 (1): 148-55AIMS: Transient receptor potential vanilloid 4 (TRPV4) may affect afferent pathways innervating the bladder. We investigated the effects of GSK1016790A (GSK) and RN1734, a TRPV4 agonist and antagonist, respectively, and P2X-purinoceptor antagonists (TNP-ATP and PPADS) on cystometry (CMG), and the effect of GSK on single afferent fiber activities (SAAs) of the rat bladder and its relationship with capsaicin (Cap)-sensitivity. METHODS: Conscious female Sprague-Dawley rats were used for CMG measurements. In SAA measurements, under urethane anesthesia, SAA was identified by electrical stimulation of the pelvic nerve and by bladder distention. Cystometric parameters were measured before and after intravesical drug instillation. In SAA measurements, response with saline instillation served as baseline. Then, GSK was instilled three times, and finally Cap was instilled to investigate the relationship with Cap-sensitivity. RESULTS: Intravesical GSK-instillation transiently decreased bladder capacity and voided volume, which were counteracted by RN1734, TNP-ATP, and PPADS. In SAA measurements, Adelta-fibers (n = 7) were not affected by either GSK or Cap. Based on the Cap-sensitivity, C-fibers could be divided into two subtypes: Cap-insensitive (n = 14) and Cap-sensitive (n = 8). In the Cap-insensitive C-fibers, GSK significantly increased the SAAs during the first instillation, but the increase attenuated with time, whereas GSK did not significantly affect the Cap-sensitive C-fibers. CONCLUSIONS: The present results suggest that activation of TRPV4 in the bladder, probably urothelium, facilitates the micturition reflex by activation of the mechanosensitive, Cap-insensitive C-fibers of the primary bladder afferents in rats.|Animals[MESH]|Capsaicin/pharmacology[MESH]|Electric Stimulation[MESH]|Female[MESH]|Leucine/analogs & derivatives/pharmacology[MESH]|Models, Animal[MESH]|Nerve Fibers, Unmyelinated/*physiology[MESH]|Neurons, Afferent/drug effects/*physiology[MESH]|Purinergic P2X Receptor Antagonists/pharmacology[MESH]|Rats[MESH]|Rats, Sprague-Dawley[MESH]|Sulfonamides/pharmacology[MESH]|TRPV Cation Channels/agonists/antagonists & inhibitors/*physiology[MESH]|Urinary Bladder/*innervation/physiology[MESH]|Urination/*physiology[MESH] |