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lüll Changes of renal vitamin D metabolic enzyme expression and calcium transporter abundance in obstructive nephropathy Zhang Y; Wu SY; Gu SS; Lv FKNephrology (Carlton) 2011[Nov]; 16 (8): 710-4The effects of urinary-tract obstruction on renal function have been clarified. However, there is little known about the change of renal vitamin D metabolic enzyme expression and vitamin D-dependent calcium transporting proteins expression in obstructive nephropathy. The male mice were subjected to unilateral ureteral obstruction (n = 10) or sham operation (n = 10). All mice were killed on day 7 after the surgical operation. Kidney sections were stained with Masson's trichrome and gene expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. The obstructed kidney exhibited interstitial fibrosis as shown by the strong collagen deposition in the interstitium. Quantitative PCR results showed the increase of 1-OHase (P < 0.001) mRNA expression and the decrease of 24-OHase (P < 0.01), CaBP-9k (P < 0.01) and CaBP-28k (P < 0.01) mRNA expression in obstructed kidney as compared to that of the Sham group. In addition, the mRNA expression of 1-OHase and CaBP-9k was significantly increased and decreased, respectively, in obstructed kidney as compared to that of the contra-lateral kidney in unilateral ureteral obstruction (UUO) mice. Together, the present finding supports the hypothesis that the ureteral obstruction leads to the alteration of renal vitamin D metabolic enzyme expression and calcium transporter abundance, which may secondarily induce the abnormality of vitamin D endocrine system and bone health.|Animals[MESH]|Calbindins[MESH]|Calcium Channels/metabolism[MESH]|Calcium-Transporting ATPases/metabolism[MESH]|Calcium/blood[MESH]|Disease Models, Animal[MESH]|Gene Expression Regulation, Enzymologic/physiology[MESH]|Kidney/enzymology[MESH]|Male[MESH]|Mice[MESH]|Mice, Inbred C57BL[MESH]|S100 Calcium Binding Protein G/*metabolism[MESH]|Steroid 21-Hydroxylase/*metabolism[MESH]|Steroid Hydroxylases/*metabolism[MESH]|TRPV Cation Channels/metabolism[MESH]|Ureteral Obstruction/*metabolism/physiopathology[MESH]|Vitamin D/*metabolism[MESH]|Vitamin D3 24-Hydroxylase[MESH] |