Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
free
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
free free
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Molecular analysis of the differentiation potential of murine mesenchymal stem cells from tissues of endodermal or mesodermal origin Nora CC; Camassola M; Bellagamba B; Ikuta N; Christoff AP; Meirelles Lda S; Ayres R; Margis R; Nardi NBStem Cells Dev 2012[Jul]; 21 (10): 1761-8Mesenchymal stem cells (MSCs) have received great attention due to their remarkable regenerative, angiogenic, antiapoptotic, and immunosuppressive properties. Although conventionally isolated from the bone marrow, they are known to exist in all tissues and organs, raising the question on whether they are identical cell populations or have important differences at the molecular level. To better understand the relationship between MSCs residing in different tissues, we analyzed the expression of genes related to pluripotency (SOX2 and OCT-4) and to adipogenic (C/EBP and ADIPOR1), osteogenic (OMD and ALP), and chondrogenic (COL10A1 and TRPV4) differentiation in cultures derived from murine endodermal (lung) and mesodermal (adipose) tissue maintained in different conditions. MSCs were isolated from lungs (L-MSCs) and inguinal adipose tissue (A-MSCs) and cultured in normal conditions, in overconfluence or in inductive medium for osteogenic, adipogenic, or chondrogenic differentiation. Cultures were characterized for morphology, immunophenotype, and by quantitative real-time reverse transcription-polymerase chain reaction for expression of pluripotency genes or markers of differentiation. Bone marrow-derived MSCs were also analyzed for comparison of these parameters. L-MSCs and A-MSCs exhibited the typical morphology, immunophenotype, and proliferation and differentiation pattern of MSCs. The analysis of gene expression showed a higher potential of adipose tissue-derived MSCs toward the osteogenic pathway and of lung-derived MSCs to chondrogenic differentiation, representing an important contribution for the definition of the type of cell to be used in clinical trials of cell therapy and tissue engineering.|*Cell Differentiation[MESH]|Abdominal Fat/*cytology[MESH]|Animals[MESH]|Antigens, CD/metabolism[MESH]|Cell Proliferation[MESH]|Cell Shape[MESH]|Cells, Cultured[MESH]|Endoderm/cytology[MESH]|Extracellular Matrix Proteins/genetics/metabolism[MESH]|Gene Expression Profiling[MESH]|Lung/*cytology[MESH]|Mesenchymal Stem Cells/metabolism/*physiology[MESH]|Mesoderm/cytology[MESH]|Mice[MESH]|Mice, Inbred C57BL[MESH]|Octamer Transcription Factor-3/genetics/metabolism[MESH]|Phenotype[MESH]|Proteoglycans/genetics/metabolism[MESH]|TRPV Cation Channels/genetics/metabolism[MESH] |