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Heteromeric TRPV4-C1 channels contribute to store-operated Ca(2+) entry in vascular endothelial cells Ma X; Cheng KT; Wong CO; O'Neil RG; Birnbaumer L; Ambudkar IS; Yao XCell Calcium 2011[Dec]; 50 (6): 502-9There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle trafficking of heteromeric TRPV4-C1 channels, causing insertion of more channels into the plasma membrane in vascular endothelial cells. In the present study, we determined whether the enhanced TRPV4-C1 insertion to the plasma membrane could contribute to SOCE and I(SOC). We found that thapsigargin-induced SOCE was much lower in aortic endothelial cells derived from trpv4(-/-) or trpc1(-/-) knockout mice when compared to that of wild-type mice. In human umbilical vein endothelial cells (HUVECs), thapsigargin-induced SOCE was markedly reduced by knocking down the expression of TRPC1 and/or TRPV4 with respective siRNAs. Brefeldin A, a blocker of vesicular translocation, inhibited the SOCE. These results suggest that an enhanced vesicular trafficking of heteromeric TRPV4-C1 channels contributes to SOCE in vascular endothelial cells. Vascular tension studies suggest that such an enhanced trafficking of TRPV4-C1 channels may play a role in thapsigargin-induced vascular relaxation in rat small mesenteric arteries.|Animals[MESH]|Brefeldin A/pharmacology[MESH]|Calcium Channels/genetics/*metabolism[MESH]|Calcium/*metabolism[MESH]|Cell Line[MESH]|Endothelial Cells/*metabolism[MESH]|Endothelium, Vascular/*metabolism[MESH]|Fura-2[MESH]|Human Umbilical Vein Endothelial Cells/drug effects/metabolism[MESH]|Humans[MESH]|Mice[MESH]|Mutation[MESH]|Patch-Clamp Techniques[MESH]|RNA, Small Interfering[MESH]|TRPC Cation Channels/genetics/*metabolism[MESH]|TRPV Cation Channels/genetics/*metabolism/physiology[MESH]|Thapsigargin/pharmacology[MESH] |
