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lüll The role of the membrane potential in chondrocyte volume regulation Lewis R; Asplin KE; Bruce G; Dart C; Mobasheri A; Barrett-Jolley RJ Cell Physiol 2011[Nov]; 226 (11): 2979-86Many cell types have significant negative resting membrane potentials (RMPs) resulting from the activity of potassium-selective and chloride-selective ion channels. In excitable cells, such as neurones, rapid changes in membrane permeability underlie the generation of action potentials. Chondrocytes have less negative RMPs and the role of the RMP is not clear. Here we examine the basis of the chondrocyte RMP and possible physiological benefits. We demonstrate that maintenance of the chondrocyte RMP involves gadolinium-sensitive cation channels. Pharmacological inhibition of these channels causes the RMP to become more negative (100 microM gadolinium: DeltaV(m) = -30 +/- 4 mV). Analysis of the gadolinium-sensitive conductance reveals a high permeability to calcium ions (PCa/PNa approximately 80) with little selectivity between monovalent ions; similar to that reported elsewhere for TRPV5. Detection of TRPV5 by PCR and immunohistochemistry and the sensitivity of the RMP to the TRPV5 inhibitor econazole (DeltaV(m) = -18 +/- 3 mV) suggests that the RMP may be, in part, controlled by TRPV5. We investigated the physiological advantage of the relatively positive RMP using a mathematical model in which membrane stretch activates potassium channels allowing potassium efflux to oppose osmotic water uptake. At very negative RMP potassium efflux is negligible, but at more positive RMP it is sufficient to limit volume increase. In support of our model, cells clamped at -80 mV and challenged with a reduced osmotic potential swelled approximately twice as much as cells at +10 mV. The positive RMP may be a protective adaptation that allows chondrocytes to respond to the dramatic osmotic changes, with minimal changes in cell volume.|Animals[MESH]|Calcium/physiology[MESH]|Cattle[MESH]|Cell Size[MESH]|Cells, Cultured[MESH]|Chondrocytes/*cytology/drug effects[MESH]|Dogs[MESH]|Gadolinium/pharmacology[MESH]|Horses[MESH]|Membrane Potentials/drug effects/*physiology[MESH]|Models, Biological[MESH]|Rats[MESH]|Sheep[MESH]|TRPV Cation Channels/drug effects/physiology[MESH] |