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lüll Combining culture techniques for Bartonella: the best of both worlds Lynch T; Iverson J; Kosoy MJ Clin Microbiol 2011[Apr]; 49 (4): 1363-8In this study we compared some common Bartonella culturing methodologies using four diverse species causing human illnesses. Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that Bartonella tamiae demonstrated temperature-dependent growth limitations between common culturing conditions only 2 degrees C apart. Additionally, growth of B. quintana was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature- and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.|Animals[MESH]|Bacteriological Techniques/*methods[MESH]|Bartonella Infections/*diagnosis/microbiology[MESH]|Bartonella/growth & development/*isolation & purification[MESH]|Cell Culture Techniques[MESH]|Cell Line[MESH]|Coculture Techniques[MESH]|Culture Media/chemistry[MESH]|Humans[MESH]|Insecta[MESH]|Temperature[MESH] |