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DNA-based methods in the immunohematology reference laboratory Reid ME; Denomme GATransfus Apher Sci 2011[Feb]; 44 (1): 65-72Although hemagglutination serves the immunohematology reference laboratory well, when used alone, it has limited capability to resolve complex problems. This overview discusses how molecular approaches can be used in the immunohematology reference laboratory. In order to apply molecular approaches to immunohematology, knowledge of genes, DNA-based methods, and the molecular bases of blood groups are required. When applied correctly, DNA-based methods can predict blood groups to resolve ABO/Rh discrepancies, identify variant alleles, and screen donors for antigen-negative units. DNA-based testing in immunohematology is a valuable tool used to resolve blood group incompatibilities and to support patients in their transfusion needs.|*Allergy and Immunology[MESH]|*Laboratories[MESH]|*Molecular Diagnostic Techniques[MESH]|Adult[MESH]|Blood Banks[MESH]|Blood Group Antigens/analysis/*genetics[MESH]|Blood Group Incompatibility/diagnosis/genetics[MESH]|Blood Grouping and Crossmatching/*methods[MESH]|Blood Transfusion[MESH]|DNA/*genetics[MESH]|Female[MESH]|Fetal Blood/chemistry[MESH]|Hematologic Diseases/blood/diagnosis/genetics[MESH]|Hematology/*methods[MESH]|Humans[MESH]|Infant, Newborn[MESH]|Informed Consent[MESH]|Pregnancy[MESH]|Pregnancy Complications/blood[MESH]|Reference Standards[MESH] |
