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Whole genome amplification in preimplantation genetic diagnosis Zheng YM; Wang N; Li L; Jin FJ Zhejiang Univ Sci B 2011[Jan]; 12 (1): 1-11Preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover, WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review, we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA products.|Alleles[MESH]|Comparative Genomic Hybridization[MESH]|DNA Mutational Analysis/methods[MESH]|DNA Primers[MESH]|Diagnostic Errors[MESH]|Female[MESH]|Haplotypes[MESH]|Humans[MESH]|Male[MESH]|Microsatellite Repeats[MESH]|Nucleic Acid Amplification Techniques/*methods[MESH]|Polymerase Chain Reaction/methods[MESH]|Polymorphism, Single Nucleotide[MESH]|Pregnancy[MESH]|Preimplantation Diagnosis/*methods[MESH] |
