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Building mitotic chromosomes Ohta S; Wood L; Bukowski-Wills JC; Rappsilber J; Earnshaw WCCurr Opin Cell Biol 2011[Feb]; 23 (1): 114-21Mitotic chromosomes are the iconic structures into which the genome is packaged to ensure its accurate segregation during mitosis. Although they have appeared on countless journal cover illustrations, there remains no consensus on how the chromatin fiber is packaged during mitosis. In fact, work in recent years has both added to existing controversies and sparked new ones. By contrast, there has been very significant progress in determining the protein composition of isolated mitotic chromosomes. Here, we discuss recent studies of chromosome organization and provide an in depth description of the latest proteomics studies, which have at last provided us with a definitive proteome for vertebrate chromosomes.|*Mitosis[MESH]|Animals[MESH]|Chromosomes/*metabolism[MESH]|Humans[MESH]|Models, Molecular[MESH] |
