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lüll Oncolytic adenovirus SG600-IL24 selectively kills hepatocellular carcinoma cell lines Xue XB; Xiao CW; Zhang H; Lu AG; Gao W; Zhou ZQ; Guo XL; Zhong MA; Yang Y; Wang CJWorld J Gastroenterol 2010[Oct]; 16 (37): 4677-84AIM: To investigate the effect of oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24 on hepatocellular carcinoma (HCC) cell lines and normal liver cell line. METHODS: HCC cell lines (HepG2, Hep3B and MHCC97L) and normal liver cell line (L02) with a different p53 status were infected with SG600-IL24 and Ad.IL-24, respectively. Melanoma differentiation-associated (MDA)-7/interleukin (IL)-24 mRNA and protein expressions in infected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and Western blotting, respectively. Apoptosis of HCC cells and normal liver cells was detected by cytometric assay with Hoechst33258 staining. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to investigate proliferation of HCC cells and normal liver cells, and cell cycle was assayed by flow cytometry. RESULTS: RT-PCR, ELISA and Western blotting showed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT indicated that SG600-IL24 could suppress the growth of HepG2, Hep3B, MHCC97L, with an inhibition rate of 75% +/- 2.5%, 85% +/- 2.0%, 72% +/- 1.8%, respectively (P < 0.01), promote the apoptosis of HepG2, Hep3B, MHCC97L, with an apoptosis rate of 56.59% +/- 4.0%, 78.36% +/- 3.5%, 43.39% +/- 2.5%, respectively (P < 0.01), and block the HCC cell lines in the G2/M phase with a blocking rate of 35.4% +/- 4.2%, 47.3% +/- 6.2%, 42% +/- 5.0%, respectively (P < 0.01) but not the normal liver cell line in a p53-independent manner. CONCLUSION: SG600-IL24 can selectively suppress the proliferation and apoptosis of HCC cell lines in vitro but not normal liver cell line L02 in a p53-independent manner. Compared with Ad.IL-24, SG600-IL24 can significantly enhance the antitumor activity in HCC cell lines.|*Interleukins/genetics/immunology[MESH]|Adenoviridae/genetics/*metabolism/pathogenicity[MESH]|Apoptosis[MESH]|Carcinoma, Hepatocellular/*virology[MESH]|Cell Line, Tumor/*virology[MESH]|Humans[MESH]|Liver Neoplasms/*virology[MESH]|Neoplasm Proteins/genetics/metabolism[MESH]|Oncolytic Viruses/genetics/*metabolism/pathogenicity[MESH] |