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Evaluating genome-scale approaches to eukaryotic DNA replication Gilbert DMNat Rev Genet 2010[Oct]; 11 (10): 673-84Mechanisms regulating where and when eukaryotic DNA replication initiates remain a mystery. Recently, genome-scale methods have been brought to bear on this problem. The identification of replication origins and their associated proteins in yeasts is a well-integrated investigative tool, but corresponding data sets from multicellular organisms are scarce. By contrast, standardized protocols for evaluating replication timing have generated informative data sets for most eukaryotic systems. Here, I summarize the genome-scale methods that are most frequently used to analyse replication in eukaryotes, the kinds of questions each method can address and the technical hurdles that must be overcome to gain a complete understanding of the nature of eukaryotic replication origins.|Animals[MESH]|DNA Replication/*genetics[MESH]|Eukaryotic Cells/*metabolism[MESH]|Genome/genetics[MESH]|Genomics/*methods[MESH]|Humans[MESH]|Models, Genetic[MESH]|Replication Origin/*genetics[MESH]|Reproducibility of Results[MESH] |
