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lüll Mcl-1 downregulation by pro-inflammatory cytokines and palmitate is an early event contributing to beta-cell apoptosis Allagnat F; Cunha D; Moore F; Vanderwinden JM; Eizirik DL; Cardozo AKCell Death Differ 2011[Feb]; 18 (2): 328-37Pancreatic beta-cell apoptosis is a key feature of diabetes mellitus and the mitochondrial pathway of apoptosis is a major mediator of beta-cell death. We presently evaluated the role of the myeloid cell leukemia sequence 1 (Mcl-1), an antiapoptotic protein of the Bcl-2 family, in beta-cells following exposure to well-defined beta-cell death effectors, for example, pro-inflammatory cytokines, palmitate and chemical endoplasmic reticulum (ER) stressors. All cytotoxic stresses rapidly and preferentially decreased Mcl-1 protein expression as compared with the late effect observed on the other antiapoptotic proteins, Bcl-2 and Bcl-xL. This was due to ER stress-mediated inhibition of translation through eIF2alpha phosphorylation for palmitate and ER stressors and through the combined action of translation inhibition and JNK activation for cytokines. Knocking down Mcl-1 using small interference RNAs increased apoptosis and caspase-3 cleavage induced by cytokines, palmitate or thapsigargin, whereas Mcl-1 overexpression partly prevented Bax translocation to the mitochondria, cytochrome c release, caspase-3 cleavage and apoptosis induced by the beta-cell death effectors. Altogether, our data suggest that Mcl-1 downregulation is a crucial event leading to beta-cell apoptosis and provide new insights into the mechanisms linking ER stress and the mitochondrial intrinsic pathway of apoptosis. Mcl-1 is therefore an attractive target for the design of new strategies in the treatment of diabetes.|*Apoptosis[MESH]|Animals[MESH]|Caspase 3/metabolism[MESH]|Cell Line, Tumor[MESH]|Cytochromes c/metabolism[MESH]|Cytokines/*pharmacology[MESH]|Down-Regulation[MESH]|Endoplasmic Reticulum/metabolism[MESH]|Insulin-Secreting Cells/cytology/*metabolism[MESH]|Myeloid Cell Leukemia Sequence 1 Protein[MESH]|Palmitates/*pharmacology[MESH]|Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism[MESH]|RNA Interference[MESH]|RNA, Small Interfering/metabolism[MESH]|Rats[MESH]|Thapsigargin/pharmacology[MESH]|bcl-2-Associated X Protein/metabolism[MESH]|bcl-X Protein/metabolism[MESH] |