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lüll Two-photon microscopy analysis of leukocyte trafficking and motility Okada TSemin Immunopathol 2010[Sep]; 32 (3): 215-25During the last several years, live tissue imaging, in particular using two-photon laser microscopy, has advanced our understanding of leukocyte trafficking mechanisms. Studies using this technique are revealing distinct molecular requirements for leukocyte migration in different tissue environments. Also emerging from the studies are the ingenious infrastructures for leukocyte trafficking, which are produced by stromal cells. This review summarizes the recent imaging studies that provided novel mechanistic insights into in vivo leukocyte migration essential for immunosurveillance.|Animals[MESH]|Cell Movement/*immunology/*physiology[MESH]|Chemotaxis, Leukocyte/physiology[MESH]|Cytoskeletal Proteins/metabolism[MESH]|Humans[MESH]|Hyaluronan Receptors/metabolism[MESH]|In Vitro Techniques[MESH]|Integrins/physiology[MESH]|Leukocytes/*immunology/*physiology[MESH]|Lymphocytes/physiology[MESH]|Microscopy, Fluorescence, Multiphoton/*methods[MESH]|Models, Biological[MESH]|Monomeric GTP-Binding Proteins/metabolism[MESH]|Receptors, Chemokine/metabolism[MESH]|Receptors, G-Protein-Coupled/metabolism[MESH]|Time-Lapse Imaging/methods[MESH]|Tissue Scaffolds[MESH] |