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TRPV4 channels augment macrophage activation and ventilator-induced lung injury Hamanaka K; Jian MY; Townsley MI; King JA; Liedtke W; Weber DS; Eyal FG; Clapp MM; Parker JCAm J Physiol Lung Cell Mol Physiol 2010[Sep]; 299 (3): L353-62We have previously implicated transient receptor potential vanilloid 4 (TRPV4) channels and alveolar macrophages in initiating the permeability increase in response to high peak inflation pressure (PIP) ventilation. Alveolar macrophages were harvested from TRPV4(-/-) and TRPV4(+/+) mice and instilled in the lungs of mice of the opposite genotype. Filtration coefficients (K(f)) measured in isolated perfused lungs after ventilation with successive 30-min periods of 9, 25, and 35 cmH(2)O PIP did not significantly increase in lungs from TRPV4(-/-) mice but increased >2.2-fold in TRPV4(+/+) lungs, TRPV4(+/+) lungs instilled with TRPV4(-/-) macrophages, and TRPV4(-/-) lungs instilled with TRPV4(+/+) macrophages after ventilation with 35 cmH(2)O PIP. Activation of TRPV4 with 4-alpha-phorbol didecanoate (4alphaPDD) significantly increased intracellular calcium, superoxide, and nitric oxide production in TRPV4(+/+) macrophages but not TRPV4(-/-) macrophages. Cross-sectional areas increased nearly 3-fold in TRPV4(+/+) macrophages compared with TRPV4(-/-) macrophages after 4alphaPDD. Immunohistochemistry staining of lung tissue for nitrotyrosine revealed increased amounts in high PIP ventilated TRPV4(+/+) lungs compared with low PIP ventilated TRPV4(+/+) or high PIP ventilated TRPV4(-/-) lungs. Thus TRPV4(+/+) macrophages restored susceptibility of TRPV4(-/-) lungs to mechanical injury. A TRPV4 agonist increased intracellular calcium and reactive oxygen and nitrogen species in harvested TRPV4(+/+) macrophages but not TRPV4(-/-) macrophages. K(f) increases correlated with tissue nitrotyrosine, a marker of peroxynitrite production.|*Macrophage Activation[MESH]|Animals[MESH]|Disease Susceptibility[MESH]|Genotype[MESH]|Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/metabolism[MESH]|Immunohistochemistry/methods[MESH]|In Vitro Techniques[MESH]|Lung/metabolism[MESH]|Macrophages, Alveolar/metabolism/pathology/transplantation[MESH]|Mice[MESH]|Mice, Knockout[MESH]|Permeability[MESH]|Phorbol Esters/pharmacology[MESH]|Pulmonary Edema/physiopathology[MESH]|Pulmonary Ventilation[MESH]|Reactive Nitrogen Species/metabolism[MESH]|Reactive Oxygen Species/metabolism[MESH]|Staining and Labeling[MESH]|TRPC Cation Channels/agonists/deficiency/*metabolism[MESH]|Tyrosine/analogs & derivatives/metabolism[MESH]|Ventilator-Induced Lung Injury/pathology/*physiopathology[MESH] |
