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Expression and immunolocalization of calcium transport proteins in the canine duodenum, kidney, and pancreas Palm C; Hartmann K; Weber KAnat Rec (Hoboken) 2010[May]; 293 (5): 770-4The aim of the study was to elucidate expression and localization of several calcium handling proteins and the regulating vitamin D receptor (VDR) in the dog using RT-PCR and immunohistochemistry. The results of RNA expression and antibody staining were compared to ensure specificity. We found several antibodies to be useful for further studies on calcium homeostasis.|Animals[MESH]|Antibodies/immunology/isolation & purification[MESH]|Antibody Specificity/immunology[MESH]|Calbindins[MESH]|Calcium Channels/genetics/*metabolism[MESH]|Calcium Signaling/*physiology[MESH]|Calcium-Transporting ATPases/genetics/*metabolism[MESH]|Calcium/metabolism[MESH]|Dogs[MESH]|Duodenum/cytology/*metabolism[MESH]|Gene Expression Regulation/physiology[MESH]|Immunohistochemistry/methods[MESH]|Kidney/cytology/*metabolism[MESH]|Pancreas/cytology/*metabolism[MESH]|RNA, Messenger/metabolism[MESH]|Receptors, Calcitriol/genetics/metabolism[MESH]|Reverse Transcriptase Polymerase Chain Reaction[MESH]|S100 Calcium Binding Protein G/genetics/metabolism[MESH]|Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics/metabolism[MESH]|Sodium-Calcium Exchanger/genetics/metabolism[MESH]|Species Specificity[MESH]|TRPV Cation Channels/genetics/metabolism[MESH] |
