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Laser desorption postionization for imaging MS of biological material Akhmetov A; Moore JF; Gasper GL; Koin PJ; Hanley LJ Mass Spectrom 2010[Feb]; 45 (2): 137-45Vacuum ultraviolet single photon ionization (VUV SPI) is a soft ionization technique that has the potential to address many of the limitations of matrix-assisted laser desorption/ionization (MALDI) for imaging MS. Laser desorption postionization (LDPI) uses VUV SPI for postionization and is experimentally analogous to a MALDI instrument with the addition of a pulsed VUV light source. This review discusses progress in LDPI-MS over the last decade, with an emphasis on imaging MS of bacterial biofilms, analytes whose high salt environment make them particularly resistant to imaging by MALDI-MS. This review first considers fundamental aspects of VUV SPI including ionization mechanisms, cross sections, quantum yields of ionization, dissociation and potential mass limits. The most common sources of pulsed VUV radiation are then described along with a newly constructed LDPI-MS instrument with imaging capabilities. Next, the detection and imaging of small molecules within intact biofilms is demonstrated by LDPI-MS using 7.87 eV (157.6 nm) VUV photons from a molecular fluorine excimer laser, followed by the use of aromatic tags for detection of selected species within the biofilm. The final section considers the future prospects for imaging intact biological samples by LDPI-MS.|*Biofilms[MESH]|Diagnostic Imaging/*instrumentation/methods/trends[MESH]|Equipment Design[MESH]|Models, Molecular[MESH]|Peptides/analysis[MESH]|Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*instrumentation/methods/trends[MESH] |