Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
free
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
free free
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll A 3 5-nm structure of rat TRPV4 cation channel revealed by Zernike phase-contrast cryoelectron microscopy Shigematsu H; Sokabe T; Danev R; Tominaga M; Nagayama KJ Biol Chem 2010[Apr]; 285 (15): 11210-8The transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel responsive to various stimuli including cell swelling, warm temperatures (27-35 degrees C), and chemical compounds such as phorbol ester derivatives. Here we report the three-dimensional structure of full-length rat TRPV4 purified from baculovirus-infected Sf9 cells. Hexahistidine-tagged rat TRPV4 (His-rTRPV4) was solubilized with detergent and purified through affinity chromatography and size-exclusion chromatography. Chemical cross-linking analysis revealed that detergent-solubilized His-rTRPV4 was a tetramer. The 3.5-nm structure of rat TRPV4 was determined by cryoelectron microscopy using single-particle reconstruction from Zernike phase-contrast images. The overall structure comprises two distinct regions; a larger dense component, likely corresponding to the cytoplasmic N- and C-terminal regions, and a smaller component corresponding to the transmembrane region.|Animals[MESH]|Calcium/chemistry[MESH]|Cell Line[MESH]|Chromatography, Affinity/methods[MESH]|Cross-Linking Reagents/chemistry[MESH]|Cryoelectron Microscopy/*methods[MESH]|Detergents/pharmacology[MESH]|Image Processing, Computer-Assisted[MESH]|Insecta[MESH]|Microscopy, Electron, Transmission/methods[MESH]|Microscopy, Phase-Contrast/*methods[MESH]|Molecular Conformation[MESH]|Protein Conformation[MESH]|Rats[MESH]|TRPV Cation Channels/*chemistry[MESH] |