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Aptamer-encoded nanopore for ultrasensitive detection of bioterrorist agent ricin at single-molecule resolution Gu LQ; Ding S; Gao CAnnu Int Conf IEEE Eng Med Biol Soc 2009[]; 2009 (ä): 6699-702The molecular-scale pore structure, called nanopore, can be formed from protein ion channels by genetic engineering or fabricated on solid substrates using fashion nanotechnology. Target molecules in interaction with the functionalized lumen of nanopore, can produce characteristic changes in the pore conductance, which act as fingerprints, allowing us to identify single molecules and simultaneously quantify each target species in the mixture. Nanopore sensors have been created for tremendous biomedical detections, with targets ranging from metal ions, drug compounds and cellular second messengers, to proteins and DNAs. Here we will review our recent discoveries with a lab-in-hand glass nanopore: single-molecule discrimination of chiral enantiomers with a trapped cyclodextrin, and sensing of bioterrorist agent ricin.|*Bioterrorism[MESH]|Aptamers, Peptide/*chemistry[MESH]|Nanostructures/*chemistry[MESH]|Nanotechnology/*methods[MESH]|Porosity[MESH]|Ricin/*analysis[MESH] |
