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RNA-seq: from technology to biology Marguerat S; Bahler JCell Mol Life Sci 2010[Feb]; 67 (4): 569-79Next-generation sequencing technologies are now being exploited not only to analyse static genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these powerful and rapidly evolving technologies have only been available for a couple of years, they are already making substantial contributions to our understanding of genome expression and regulation. Here, we briefly describe technical issues accompanying RNA-seq data generation and analysis, highlighting differences to array-based approaches. We then review recent biological insight gained from applying RNA-seq and related approaches to deeply sample transcriptomes in different cell types or physiological conditions. These approaches are providing fascinating information about transcriptional and post-transcriptional gene regulation, and they are also giving unique insight into the richness of transcript structures and processing on a global scale and at unprecedented resolution.|*Gene Expression Regulation[MESH]|*Transcription, Genetic[MESH]|Animals[MESH]|Humans[MESH]|Mice[MESH]|Oligonucleotide Array Sequence Analysis/*methods[MESH]|Sequence Analysis, RNA/*methods[MESH] |
