Warning: Undefined variable $zfal in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 525
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 530
Warning: Undefined variable $sterm in C:\Inetpub\vhosts\kidney.de\httpdocs\mlpefetch.php on line 531
English Wikipedia
Nephropedia Template TP (
Twit Text
DeepDyve Pubget Overpricing |
lüll Odontoblast TRP channels and thermo/mechanical transmission Son AR; Yang YM; Hong JH; Lee SI; Shibukawa Y; Shin DMJ Dent Res 2009[Nov]; 88 (11): 1014-9Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.|Animals[MESH]|Animals, Newborn[MESH]|Ankyrin Repeat[MESH]|Calcium Channels/analysis[MESH]|Calcium Signaling/physiology[MESH]|Cells, Cultured[MESH]|Cold Temperature[MESH]|Dental Pulp/cytology[MESH]|Hot Temperature[MESH]|Mechanoreceptors/physiology[MESH]|Mechanotransduction, Cellular/*physiology[MESH]|Mice[MESH]|Mice, Inbred ICR[MESH]|Odontoblasts/*physiology[MESH]|Osmosis[MESH]|Signal Transduction/*physiology[MESH]|TRPA1 Cation Channel[MESH]|TRPM Cation Channels/analysis[MESH]|TRPV Cation Channels/analysis[MESH]|Thermosensing/*physiology[MESH]|Transient Receptor Potential Channels/analysis/*physiology[MESH] |